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. 2020 Oct 21;10:17937. doi: 10.1038/s41598-020-73908-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of all iPSC-LBs using defined medium (Step VI). (a) Morphological representation of all iPSC-LBs generated using cells differentiated in the CD-AOF medium. Green segments in the bottom panel represent successful recognition of individual LBs in dimple-well plates. Scale bar, 200 µm. (b) Morphological analysis of the LBs generated using cells differentiated in the CD-AOF medium. (c) Gene expression analysis of the LBs generated using cells differentiated in the conventional or CD-AOF medium. Error bars represent standard deviation. Bars with the different letters are significantly different according to one-way ANOVA (analysis of variance) with post-hoc Tukey HSD (honestly significant difference) test (p < 0.05). See supplementary note for detailed interpretations. PHH: primary human hepatocytes. Liver: human liver tissue. (d) Human albumin secretion in iPSC-LBs differentiated in the conventional or CD-AOF medium.