Table 2.

Effect of in utero GEN exposure on body weight, absolute and relative spleen weight in middle-aged B6C3F1 offspring

PND		Parameters	Naïve	Vehicle	Genistein
Female	240	Body wt (g)	33.43 ± 1.88	35.12 ± 2.59	33.93 ± 1.74
			(7)	(6)	(7)
		Spleen (mg)	81.25 ± 2.46	105.50 ± 8.79 a	87.86 ± 2.76 b
		%Body Wt	0.244 ± 0.014	0.307 ± 0.030	0.263 ± 0.018
290		Body wt (g)	43.49 ± 2.38	41.02 ± 1.59	39.91 ± 1.96
			(8)	(6)	(7)
		Spleen (mg)	106.88 ± 8.82	113.83 ± 5.64	98.14 ± 5.32
		%Body Wt	0.247 ± 0.016	0.279 ± 0.017	0.253 ± 0.025
330		Body wt (g)	42.16 ± 2.06	40.97 ± 2.74	36.43 ± 2.42
			(8)	(6)	(7)
		Spleen (mg)	105.50 ± 5.27	103.17 ± 2.65	126.29 ± 8.74 b
		%Body Wt	0.253 ± 0.016	0.258 ± 0.018	0.356 ± 0.032 a,b
Male	240	Body wt (g)	44.48 ± 0.97	41.79 ± 0.30 a	42.17 ± 0.72
			(8)	(8)	(8)
		Spleen (mg)	89.63 ± 5.15	80.63 ± 1.59	80.25 ± 2.85
		%Body Wt	0.201 ± 0.009	0.193 ± 0.005	0.190 ± 0.006
290		Body wt (g)	46.25 ± 1.29	43.98 ± 0.91	43.34 ± 0.46 a
			(6)	(8)	(8)
		Spleen (mg)	99.17 ± 5.84	90.25 ± 6.26	95.25 ± 3.00
		%Body Wt	0.214 ± 0.008	0.204 ± 0.011	0.220 ± 0.006
330		Body wt (g)	47.66 ± 1.18	44.25 ± 0.67 a	43.29 ± 0.87 a
			(8)	(8)	(8)
		Spleen (mg)	113.13 ± 9.86	110.13 ± 10.25	105.88 ± 5.73
		%Body Wt	0.236 ± 0.016	0.249 ± 0.023	0.244 ± 0.010

Pregnant mice were treated with genistein or vehicle from GD14 to birth by gavage. Female or male offspring were sacrificed at three different time points (PND 240, 290, and 330) following HDM dosing, and their body weight and spleens were weighed. Naive = untreated, non-sensitized naïve animals.

^a,significantly different from naive;

^b,significantly different from vehicle. The number in parenthesis indicates the number in each group.