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. 2020 Oct 21;10:17929. doi: 10.1038/s41598-020-72041-3

Figure 3.

Figure 3

Neonicotinoids disrupt sleep in honey bee foragers. Four day average sleep profiles for control bees and bees exposed to either (A) thiamethoxam (Two-way RM ANOVA, Time p < 0.0001, ****; Dose p = 0.0004, ***; Interaction p = 0.0015, **) or (B) clothianidin (Two-way RM ANOVA, Time p < 0.0001, ****; Dose p = 0.0054, **; Interaction p < 0.0001, ****). Significant decreases in total sleep duration for exposure to (C) thiamethoxam (One-way ANOVA F = 6.436; p = 0.0004, ***; Dunnett’s multiple comparison 70 ppb p = 0.0038, **; 140 ppb p = 0.0026, **) or exposure to (D) clothianidin (One-way ANOVA F = 4.205; p = 0.0067, **; Dunnett’s multiple comparison 140 ppb p = 0.0115, *). In addition, there were significant decreases in the number of sleep episodes for exposure to (E) thiamethoxam (One-way ANOVA F = 3.020; p = 0.0314*; Dunnett’s multiple comparison 140 ppb p < 0.0233, *) and for (F) clothianidin (One-way ANOVA F = 2.586; p = 0.0549, ns; Dunnett’s multiple comparison 140 ppb p < 0.0396, *) dosed bees. Significant values only are shown for Dunnett’s post hoc multiple comparisons (CF).