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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: Toxicol Lett. 2020 Aug 9;333:303–311. doi: 10.1016/j.toxlet.2020.08.003

Figure 1: Menthol flavor pod exposure alter mitochondrial respiration in lung epithelial cells immediately and 24-hours post-exposure.

Figure 1:

Figure 1:

Beas2b cells with 20,000 cells per seahorse well were grown to 90% confluency. Immediately after serum deprivation, the wells were exposed to a three-session exposure to JUUL Menthol pods with 12-hour intervals between sessions. (A) Immediate post final exposure graphs of oxygen consumption rate and extracellular acidification rate. (B) Immediate post final exposure values for mitochondrial respiration. ** p < 0.01, or *** p < 0.001 vs air exposed, unpaired t-test N = 6 wells per group. (C) 24 hours post final exposure graphs of oxygen consumption rate and extracellular acidification rate. (D) 24 hours post final exposure values for mitochondrial respiration. *** p < 0.001 vs air exposed, unpaired t-test N = 6 wells per group.