KEY RESOURCES TABLE.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Mycobacterium tuberculosis containing cos allele of dnaA (Mtbcos)	[19]	N/A
Mycobacterium tuberculosis H37Rv (MtbRv)	C. Sassetti	N/A
Mtbcos containing fhaA-mVenus	This paper	N/A
MtbRv containing fhaA-mVenus	This paper	N/A
Mycobacterium smegmatis MC2155 (Msm)	C. Sassetti	N/A
Msm containing ftsW mVenus and divIVA-RFP	This paper	N/A
Msm smegmatis containing pknD-mVenus and divIVA-RFP	This paper	N/A
Mtb guaB2-DAS-HygR+Giles-TetON1-sspB-strR	[71]	N/A
Mtb gyrB-DAS-HygR+Giles-TetON6-sspB-strR	[71]	N/A
Mtb pyrE-DAS-HygR+Giles-TetON1-sspB-strR	[71]	N/A
Msm containing m-Venus pMV261-HygR	Eric Rubin Lab	N/A
Chemicals, Peptides, and Recombinant Proteins
VCC234718	[43]	N/A
C109	[50]	N/A
Guanine-98%	Millipore-Sigma	G11950
Guanosine > = 98%	Millipore-Sigma	G6264
Spectinomycin dihydrochloride pentahydrate	Millipore-Sigma	S4014
Critical Commercial Assays
KAPA Stranded RNA-Seq Kit	Roche	KK8401
Ribo-Zero Bacteria Kit (discontinued)	Illumina	MRZB12424
iQ SYBR Green Supermix	Bio-Rad	1708880
Deposited Data
RNASeq count data	This paper	GEO: GSE147345
Oligonucleotides
Forward Primer for amplifying ori- 5’-GGTTCAGGCTTCACCACAGT-3’	This paper	N/A
Reverse Primer for amplifying ori- 5’-GGAGCGCTGAGATTAGCATC-3’	This paper	N/A
Forward Primer for amplifying ter- 5’-ACAACGAGAAACCGCAAATC-3’	This paper	N/A
Reverse primer for amplifying ter- 5’-TACGGCTGTCATGTCTTTCG-3’	This paper	N/A
Recombinant DNA
fhaA-mVenus in plasmid MEH	[20]	N/A
divIVA-RFP in plasmid MCtH	[57]	N/A
pknD-mVenus in plasmid MEK	[72]	N/A
Software and Algorithms
CellProfiler™	[73]	N/A
DESeq2	[74]	R package
Source code for GP smoothing, curve fitting and cell cycle modelling	https://github.com/ioerger/synchronized_cells	Python and R scripts