Extended Data Fig. 1. Notch4 expression on lung T_reg cells in allergic airway inflammation.

a-c, Flow cytometric analysis, cell frequencies and (MFI) of Notch1, 2 and 3 expression on lung T_reg and T_eff cells in Foxp3^YFPCre (n=5). d, Cell frequencies of Notch4 expression on OT-II⁺CD4⁺Foxp3⁺ T cells generated in co-cultures with sham or OVA_323–339+UFP-pulsed alveolar macrophages without or with IL-1β, IL-25, IL-33, TSLP or TNF (n=5). e, ChIP assays for the binding of STAT3 and control (IgG) antibodies to the Notch1, 2 and 3 promoters in lung T_reg cells of OVA+UFP-treated Foxp3^YFPCre, and Foxp3^YFPCreStat3^Δ/Δ mice (n=5). Each symbol represents one mouse. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: One-way ANOVA with Dunnett’s post hoc analysis (a-c); two-way ANOVA with Sidak’s post hoc analysis (d,e). **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.