FIG. 2.

Survival, hatching, and morphological defect rates for casper embryos exposed to low NaOCl or high NaOCl surface disinfection protocols. (A) Data from 6 HPF casper embryos exposed to EM alone during surface disinfection. (B) Data from 6 HPF casper embryos exposed to MethB+EM during surface disinfection. (C) Data from 24 HPF casper embryos exposed to EM alone during surface disinfection. (D) Data from 24 HPF casper embryos exposed to MethB+EM during surface disinfection. Statistical significance of survival or hatching when experimental groups are compared with control groups are indicated by *. Groups with and without MethB were evaluated separately (*p < 0.0001, Fisher's exact test). a, b, c, d, e, f, g, and h with bars denotes statistical significance when low NaOCl in EM alone is compared with experimental groups with and without MethB (a: p = 0.03 for survival and p = 0.0003 hatching, b: p = 0.0056 for hatching, c: p = 0.02 for hatching, d: p = 0.0008 for hatching, e: p < 0.0001 for hatching, f: p = 0.004 for hatching, g: p = 0.0007 for survival and p < 0.0001 for hatching, and h: p < 0.0001 for survival, Fisher's exact test). i and j with bars denotes statistical significance when high NaOCl in EM alone is compared with experimental groups with and without MethB (i: p = 0.01 for survival and p = 0.02 for hatching, and j: p = 0.02 for survival, Fisher's exact test).