Percentage of embryo survival, hatching, and morphological defects for AB and casper embryos exposed to low NaOCl or high NaOCl surface disinfection protocols. (A) Data from 6 HPF embryos exposed to EM alone during surface disinfection. (B) Data from 6 HPF embryos exposed to MethB+EM during surface disinfection. (C) Data from 24 HPF embryos exposed to EM alone during surface disinfection. (D) Data from 24 HPF embryos exposed to MethB+EM during surface disinfection. a, b, c, d, e, f, g, h, i, j, and k denote statistical significance between the respective experimental groups and the two strains (a: p < 0.001, b: p ≤ 0.0001, c: p = 0.01, d: p = 0.002, e: p = 0.0008, f: p = 0.0003, g: p = 0.003, h: p = 0.006, i: p = 0.0002, j: p = 0.03 and k: p = 0.0067, Fisher's exact test).