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. 2020 Oct 8;7:588592. doi: 10.3389/fvets.2020.588592

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Copyright © 2020 Ferrini, Mecozzi, Corsi, Ragionieri, Donofrio and Stellari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gas vs. injectable anesthesia: histological outcomes and in vivo parameters. (A) Four representative images of Masson's trichrome stained sections of vehicle and BLM lungs (scale bar, 200 μm) 21 days after the first OA administration. (B) Ashcroft score variations in vehicle and BLM mice lungs comparing the two protocols of anesthesia. Changes in BLM groups were compared to vehicle using 2-way ANOVA followed by Sidak's test for multiple comparison; ****p < 0.0001. (C) Good Spearman correlations were found between histological Ashcroft score and % hypo-aerated tissue for Iso and Alfa+Dex protocols.