TABLE 1.
Orai1 expression in the heart.
| Interfering methods | Techniques | Expression level | Antibody | Species | Cell type/tissue | Proposed location | References |
| MO-Orai1/siRNA | qRT-PCR | mRNA/Protein | Santa Cruz Biotech. (KD validated) | Zebrafish | One-cell embryos | PM | Volkers et al., 2012 |
| WB | Rat | Neonatal cardiomyocytes | |||||
| Mouse | Embryonic hearts | ||||||
| IHC | Adult hearts | ||||||
| PLA | Isolated adult cardiomyocytes | ||||||
| siRNA | qRT-PCR/WB/IC | mRNA/Protein | N/A (KD validated) | Rat | Neonatal cardiomyocytes | Perinuclear and cytosolic | Voelkers et al., 2010 |
| RNAi | RT-PCR/WB | mRNA/Protein | Millipore | Mouse | HL-1 cell line | Touchberry et al., 2011 | |
| Cardiomyocyte-specific expression dn-Orai1R91W | qRT-PCR/WB/IC/IHC | mRNA/Protein | Sigma-Aldrich (08264) (Kl and KO validated) | Mouse | Adult Hearts/Isolated adult cardiomyocytes | PM/IDs/nuclei | Bartoli et al., 2020 |
| qRT-PCR/WB | mRNA/Protein | Sigma-Aldrich (08264) | Rat | Isolated adult cardiomyocytes | Bartoli et al., 2019 | ||
| siRNA/dn-Orai1G98A Myc-tagged Orai1/Myc-tagged Orai1S34A | qRT-PCR/WB/IC/IP | mRNA/Protein | Alomone (ACC-062) (KD validated) | Human | hESC-CMs/Neonatal cardiomyocytes | Puncta along the PM/nuclei | Wang et al., 2015 |
| Rat | |||||||
| BTP2 | qRT-PCR/WB/IC | mRNA/Protein | Sigma-Aldrich (08264)/ProSci (PM-5207) | Mouse | SAN tissue and cells/Atria/Ventricles tissue | PM | Liu et al., 2015 |
| 2-APB (as activator) | WB/IHC | Protein | ProSci (4281)/ProSci (4217) | Rat | Left atria tissue/Papillary muscle | Puncta | Wolkowicz et al., 2011 |
| 2-APB (as activator) | WB/IHC | Protein | ProSci (4281)/ProSci (4217) | Rat | Left ventricles tissue | Diffuse/Puncta | Wang et al., 2012 |
| siRNA | qRT-PCR/WB/Co-IP | mRNA/Protein | Santa Cruz Biotech. (sc-68895)(KD validated) | Rat | Isolated adult cardiomyocytes | Saliba et al., 2015 | |
| siRNA | RT-PCR/WB/IC/Co-IP | mRNA/Protein | N/A (KD validated) | Human | Cardiac c-kit+ progenitor cells | PM | Che et al., 2015 |
| dnOrai1E106A/S66/BTP2 | qRT-PCR/WB/IC/Co-IP | mRNA/Protein | Sigma-Aldrich (08264) | Rat | Neonatal cardiomyocytes | Sabourin et al., 2016 | |
| Orai1+/– | WB | Protein | Mouse | Adult heart tissue | Horton et al., 2014 | ||
| WB | Protein | Rat | Isolated adult cardiomyocytes | Dominguez-Rodriguez et al., 2015 | |||
| dn-Orai1R93W | qRT-PCR | mRNA | Mouse | Heart tissue | Maus et al., 2017 | ||
| qRT-PCR | mRNA | Chicken | Heart tissue | Li et al., 2017 | |||
| BTP2/dn-Orai1E106Q | qRT-PCR/IC | mRNA/Protein | Cat | Isolated adult cardiomyocytes | PM | Troupes et al., 2017 | |
| GSK7579A/S66 | WB/IC | Protein | Alomone (ALM-25) | Mouse | Heart tissue/Isolated adult cardiomyocytes | IDs | Bonilla et al., 2019 |
| WB | Protein | Millipore | Mouse | Ventricle tissue | Correll et al., 2015 | ||
| IC | Protein | Mouse | Isolated adult cardiomyocytes | Nucleoplasm | Lee et al., 2018 | ||
| WB/IC | Protein | Cell signaling (#1280) | Rat | Heart tissue/Neonatal cardiomyocytes | Membrane fraction Puncta along the PM | Zhu-Mauldin et al., 2012 | |
| WB/Co-IP | Santa Cruz Biotech. | Heart tissue | Lu et al., 2017 | ||||
| WB | Protein | ProSci (4281) | Mouse | Isolated adult cardiomyocytes | Collins et al., 2014 | ||
| WB/Co-IP | Protein | Abcam (sc-377281) | Rat | Neonatal cardiomyocytes | Malette et al., 2019 | ||
| CRACM1+/+-LacZ | LacZ staining | Protein | Mouse | Neonatal heart | Vig et al., 2008 | ||
| siRNA | WB | Protein | Sigma-Aldrich (08264) (KD validated) | Mouse | Heart tissue | Zheng et al., 2017 | |
| Rat | Neonatal cardiomyocytes | ||||||
| qRT-PCR/WB/IHC | mRNA/Protein | Abcam | Mouse | Heart tissue/Neonatal cardiomyocytes | Dai et al., 2018 | ||
| Orai1Flox/Flox/α-MHC/Cre | qRT-PCR/IC | mRNA/Protein | N.A. (#1003) (KO validated) | Mouse | Isolated embryonic cardiomyocytes Isolated adult cardiomyocytes | Segin et al., 2020 | |
| qRT-PCR | mRNA | Human | Left ventricle tissue | Cendula et al., 2019 | |||
| IHC/IC | Protein | Alomone | Mouse | SAN/SANCs | PM | Zhang et al., 2015 | |
| WB | Protein | Santa Cruz Biotech (sc-68895) | Mouse | HL-1 cell line | Shiou et al., 2019 | ||
| RT-PCR/WB/Co-IP | mRNA/Protein | Human | Atria tissue | Zhang et al., 2013 | |||
| qRT-PCR | mRNA | Ground squirrels | Papillary muscles | Nakipova et al., 2017 | |||
| Northern-blot | RNA | Mouse | Heart tissue | Gross et al., 2007 | |||
| WB | Protein | Abcam | Rat | Neonatal cardiomyocytes | Ji et al., 2017 | ||
| RT-PCR | mRNA | Mouse | Heart tissue | Takahashi et al., 2007 | |||
| siRNA | PCR-based micro-array/WB/PLA | Protein | Novus Biologicals (KD validated) | Rat | Neonatal cardiomyocytes/Heart tissue | Dominguez-Rodriguez et al., 2018 | |
| IHC | Protein | mAb266.1/ProSci (4281) | Rat | Heart tissue | Guzman et al., 2014 | ||
| Human | |||||||
| WB | Protein | Sigma-Aldrich (08264) | Rat | Left and right ventricles tissues | Sabourin et al., 2018 |
WB, Western-blot; IC, immunocytochemistry; IHC, immunohistochemistry; PLA, proximity ligation assay; IP, immunoprecipitation; Dn, dominant negative; hESC-CMs, human embryonic stem cell-derived cardiomyocytes; PM, plasma membrane; IDs, intercalated disks.