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. 2020 Oct 8;8:582695. doi: 10.3389/fcell.2020.582695

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Copyright © 2020 Yang, Zhu, Wang, Liu, Zhou and Qian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STK35 knockdown inhibits viability and glycolysis but promotes apoptosis in SW620 and HCT116 cells. SW620 and HCT116 cells were transduced with STK35 shRNAs (shSTK35-1 and shSTK35-2) or control scramble shRNA (shNC). (A,B) Viability of (A) SW620 and (B) HCT116 cells assessed by CCK-8. (C,D) Assessment and quantification of apoptosis by flow cytometry. (E) Cellular LDH levels measured by biochemical analysis. (F,I) Energy metabolism of (F,G) SW620 and (H,I) HCT116 cells reflected by (F,H) oxygen consumption rate and (G,I) extracellular acidification rate. ***P < 0.001, compared with shNC.