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. 2020 Oct 8;8:582695. doi: 10.3389/fcell.2020.582695

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Copyright © 2020 Yang, Zhu, Wang, Liu, Zhou and Qian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STK35 overexpression promotes viability and glycolysis but inhibits apoptosis in SW480 cells through the AKT signaling pathway. SW480 cells were transduced with STK35 overexpressing lentivirus (STK35) or blank lentivirus (Vector) in the presence of 10 μM AKT inhibitor MK-2206 or control (Vehicle). (A) Cell viability assessed by CCK-8. (B,C) Cell apoptosis assessed and quantified by flow cytometry. (D) Cellular LDH levels measured by biochemical analysis. (E,F) Cellular energy metabolism reflected by (E) the oxygen consumption rate and (F) the extracellular acidification rate. (G,H) Cellular protein levels of cleaved caspase-3, cleaved caspase-9, GLUT1, HK-2, p-AKT, and AKT assessed by Western blot, with β-actin as the loading control. *P < 0.05, ***P < 0.001, compared with Vector + Vehicle, ^###P < 0.001, compared with STK35 + Vehicle.