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. 2020 Oct 8;8:582695. doi: 10.3389/fcell.2020.582695

FIGURE 6.

FIGURE 6

STK35 expression influences chemosensitivity to 5-FU. (A–D) SW620 cells stably expressing STK35 shRNAs (shSTK35) or control scramble shRNA (shNC), and SW480 cells transduced with STK35-overexpressing lentivirus (STK35) or blank lentivirus (Vector) were treated with 5-FU (0, 200, and 400 μM) or control (Vehicle) for 48 h. (A,B) SW620 and (C,D) SW480 apoptosis assessed and quantified by flow cytometry. (E–I) SW480 cells transfected with STK35-overexpressing lentivirus (STK35) or blank lentivirus (Vector) following 5-FU chemotherapy were subcutaneously injected into male nude mice (n = 6 per group). (E) Tumor volumes measured every third day, from days 12 to 33. (F) Mouse tumor characteristics, including morphology and weight, recorded at day 33. (G) Visualization of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) labeled xenograft mouse tumors. (H) Quantification of TUNEL positive cells. (I) Mice survival probability. *P < 0.05, **P < 0.01, ***P < 0.001.