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. 2020 Jul 21;6(4):308–319. doi: 10.1002/cjp2.176

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© 2020 The Authors. The Journal of Pathology: Clinical Research published by The Pathological Society of Great Britain and Ireland and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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CLEC14A protein expression in cynomolgus macaque tissues. (A) Flow cytometric analysis of CRT3 staining of human and cynomolgus macaque CLEC14A‐expressing HEK293T cells. Mean fluorescence intensity (MFI) was normalised by dividing by the MFI of wild type HEK293Tcells stained at the same antibody concentration. (B) Optimisation of immunohistochemical staining for CLEC14A in frozen human placenta sections using the CRT3 monoclonal antibody. Representative images shown. (C) Images of CD31 and CLEC14A staining on frozen sections of human placenta and cynomolgus macaque tissues. Concentration‐matched isotype control antibody staining was negative. All scale bars = 100 μm.