Table 1.
WHO RSV External Quality Assessment (EQA), 2016, for pilot laboratories’ performance in the molecular detection of RSV: Panel composition, expected and reported results
| RSV | Sample number | Duplicate samples | Expected Ct values | Expected result | Reported Ct values | Final EQA Results of 3 Laboratories scoring < 100% (N = 3/13) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Group_Strain Name | Mean (range) | Mean (range) | Laboratory 6 | Laboratory 10 | Laboratory 13 | ||||||||
| RSV | RNP | CDC rRT‐PCR | Commercial/LDT PCR | CDC rRT‐PCR | Commercial/LDT PCR | CDC rRT‐PCR | Commercial/LDT PCR | CDC rRT‐PCR | Commercial/LDT PCR | ||||
| A_Strain Long | 1 | ≤32 | ≤33 | RSV Pos | 31.26 (27.2‐35.8) | 31.46 (29.0‐31.5) | Correct | NP | Correct | Correct | Correct | Correct | |
| A_Strain Long | 2 | 2 = 8 | ≤30 | ≤34 | RSV Pos | 28.38 (27.5‐31.5) | 29.36 (25.7‐33.0) | Correct | NP | Correct | Correct | Correct | Correct |
| A_Strain Long | 3 | ≤26 | ≤34 | RSV Pos | 23.38 (22.1‐26.6) | 24.34 (21.5‐28.0) | Correct | NP | Correct | Correct | Correct | Correct | |
| Blank | 4 | Neg | ≤33 | RSV Neg | NA | NA | Correct | NP | Correct | Correct | Correct | Correct | |
| B_Strain 209_GB3 | 5 | ≤28 | ≤31 | RSV Pos | 27.71 (25.1‐31.1) | 28.24 (20.1‐32.2) | Correct | NP | Correct | Correct | Correct | Correct | |
| B_Strain 209_GB3 | 6 | ≤32 | ≤29 | RSV Pos | 32.18 (28.6‐34.1) | 34.73 (34.0‐35.4) | Correct | NP | Correct | Correct | Correct | Incorrect c | |
| B_Strain 209_GB3 | 7 | 7 = 13 | ≤35 | ≤33 | RSV Pos | 33.25 (27.5‐35.8) | 36.00 (35.0‐37.0) | Correct | NP | Correct | Correct | Correct | Incorrect c |
| A_Strain Long | 8 | 8 = 2 | ≤30 | ≤34 | RSV Pos | 29.12 (27.1‐31.3) | 29.98 (29.0‐ 33.0) | Correct | NP | Correct | Correct | Correct | Correct |
| Blank | 9 | Neg | ≤33 | RSV Neg | NA | NA | Correct | NP | Incorrect b | Incorrect b | Correct | Correct | |
| A_Strain 15353_ON1 | 10 | ≤37 | ≤33 | RSV Pos | 34.36 (29.1‐37.1) | 35.78 (27.9‐41.4) | Correct | NP | Correct | Correct | Correct | Correct | |
| A_Strain 15353_ON1 | 11 | 11 = 18 | ≤29 | ≤33 | RSV Pos | 27.52 (23.1‐30.0) | 35.78 (27.9‐41.4) | Correct | NP | Correct | Correct | Correct | Correct |
| A_Strain 15353_ON1 | 12 | ≤27 | ≤33 | RSV Pos | 24.52 (22.1‐28.2) | 25.34 (20.9‐28.0) | Correct | NP | Correct | Correct | Correct | Correct | |
| B_Strain 209_GB3 | 13 | 13 = 7 | ≤35 | ≤33 | RSV Pos | 32.31 (28.0‐35.1) | 25.34 (20.9‐28.0) | Correct | NP | Correct | Correct | Correct | Incorrect c |
| Blank | 14 | Neg | ≤33 | RSV Neg | NA | NA | Incorrect a | NP | Incorrect b | Incorrect b | Correct | Correct | |
| B_Strain 53530_BA | 15 | 15 = 20 | ≤26 | ≤31 | RSV Pos | 24.09 (21.0‐27.5) | 25.82 (18.8‐29.8) | Correct | NP | Correct | Correct | Correct | Correct |
| B_Strain 53530_BA | 16 | ≤28 | ≤31 | RSV Pos | 28.48 (24.5‐31.3) | 29.12 (23.1‐33.0) | Correct | NP | Correct | Correct | Correct | Correct | |
| B_Strain 53530_BA | 17 | ≤34 | ≤32 | RSV Pos | 31.82 (24.2‐37.4) | 32.70 (26.9‐36.3) | Correct | NP | Correct | Correct | Correct | Correct | |
| A_Strain 15353_ON1 | 18 | 18 = 11 | ≤ 29 | ≤ 33 | RSV Pos | 26.88 (23.3‐30.0) | 28.40 (25.4‐29.9) | Correct | NP | Correct | Correct | Correct | Correct |
| Blank | 19 | Neg | ≤34 | RSV Neg | NA | NA | Correct | NP | Correct | Correct | Correct | Correct | |
| B_Strain 53530_BA | 20 | 20 = 15 | ≤26 | ≤31 | RSV Pos | 24.33 (23.0‐26.2) | 26.14 (20.9‐28.4) | Correct | NP | Correct | Correct | Correct | Correct |
Pos, positive; NA, not applicable; Neg, negative; NP, not performed.
a
Reported as positive.
b
Reported as inconclusive.
c
Reported as negative.