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. 2020 Oct 8;8:582715. doi: 10.3389/fcell.2020.582715

FIGURE 6.

FIGURE 6

VC can protect oocytes from abnormal epigenetic alterations caused by MC-LR exposure. (A–C) Representative images of H3K4me2, H3K4me3, and H3K36me3 in the control, MC-LR-exposed, and VC-rescued groups. Green, H3K4me2, H3K4me3, or H3K36me3; Blue, chromosomes. Bar = 5 μm. Quantitative analysis of the fluorescence intensity of H3K4me2, H3K4me3, and H3K36me3 were recorded in graphs. (D) Relative mRNA levels of methyltransferases and demethylases related to histone methylation markers (ASH1L, NSD1, MLL2, SETD2, KDM5B, and KDM4A). *Indicates significant difference at p < 0.05 level, **p < 0.01 level, and ***p < 0.001 level.