FIGURE 4.

Effect of EW proteins on the membrane depolarization of S. Enteritidis. S. Enteritidis cells were charged with diSC₃(5) dye and incubated at 30 or 45°C in Hepes (negative control, 5 mM pH 7.2), melittin (positive control, 15 μg/mL in 5 mM Hepes, pH 7.2) and EW, EWMM, or EWF at pH 9.3. Results are shown as means of relative fluorescence units (RFU) of triplicate experiments with standard deviations. Results obtained under the conditions indicated were compared using analysis of variance and the average comparison test using the R software. Letters were used to identify average significantly different (p ≤ 0.05).