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. 2020 Sep;12(9):4771–4780. doi: 10.21037/jtd-20-203

Figure 3.

Figure 3

Directional interaction between KCNQ1OT1 and miR-2054, miR-2054 and AKT3. (A) The complementary sequence between KCNQ1OT1 and miR-2054 was obtained from the online program starbase v2.0 (http://starbase.sysu.edu.cn/). (B) Overexpression of KCNQ1OT1 and miR-2054 in cardiomyocytes transfected by recombinant adenovirus. (C) Perform a luciferase reporter gene test to detect luciferase activity at the binding site on KCNQ1OT1 when miR-2054 is overexpressed. (D) RNA pull-down test showed that lncRNA KCNQ1OT1 can be enriched by biotin-labeled miR-2054, but not by biotin-labeled miR-2054 mutant. (E) Spearman correlation analysis was used to analyze the correlation between KCNQ1OT1 level and miR-2054 level in the TAC group. (F) The complementary sequence between miR-2054 and AKT3 was obtained from Targetscan Human 7.2 (http://www.targetscan.org/). (G) Luciferase reporter assay is conducted to detect the luciferase activity of the binding site on AKT3. (H) The correlation between the levels of miR-2054 and AKT3 in the TAC group was analyzed by Spearman correlation analysis. *, P<0.05. TAC, transverse aortic constriction.