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Pi Induces Atf3 Expression, Which Is Attenuated by miR-27a-3p
(A and B) Pi (6 days) increased the Atf3 transcript amount as determined by quantitative RT-PCR (A) or by western blot analysis (B). (C) The Atf3 transcript amount was gradually increased by Pi in a time-dependent fashion. (D) Atf mRNA was also increased by vitamin D3 administration in mice. Numbers in bars represent the numbers of mice tested. Error bars indicate SD. ∗p < 0.05, ∗∗p < 0.01.
