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. 2020 Oct 20;6(10):e05302. doi: 10.1016/j.heliyon.2020.e05302

Table 1.

Single-factor experiment design of enzymolysis.

Protein Level Factors
T (°C) S: L1 E/S2 (%) t (h) pH
Chicken liver 1 45 4:1 0.4 1 6
2 50 3:1 0.6 2 6.5
3 55 2:1 0.8 3 7
4 60 1:1 1 4 7.5
5 65 1:2 1.2 5 8
L. edodes 1 40 1:2 0.2 1 4
2 45 1:4 0.4 2 4.5
3 50 1:6 0.6 3 5
4 55 1:8 0.8 4 5.5
5 60 1:10 1 5 6
T. molitor 1 45 1:3 0.4 2 7
2 50 1:6 0.8 3 7.5
3 55 1:9 1.2 4 8
4 60 1:12 1.6 5 8.5
5 65 1:15 2 6 9
1

Smashed protein material: Liquid (water).

2

Enzyme/Smashed protein material ∗ 100 %. Otherwise, enzymes used for hydrolysis of chicken liver refer to Nikolaev et al. [13], that of L. edodes refer to Lotfy et al. [14], that of T. molitor refer to Lu et al. [15].