Fig. 5. Effect of colchicine (1) and compounds (5c) and (5i) on mitotic spindle formation and β-tubulin expression in pancreatic epithelial cells COLO-357. COLO-357 cells (seeded at 105/glass) were incubated for 72 h without (a) or with 5 μM of colchicine (1) (b), 5c (c), or 5c (d), fixed, stained with anti-βtubulin antibody (red) and nuclei stain Hoechst 33342 (blue). Scale bars correspond to 16–18 μm.
