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. 2020 Oct 13;2020:8855585. doi: 10.1155/2020/8855585

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Copyright © 2020 Justina Kamarauskaite et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Original curves of kidney mitochondrial respiration in the control and ischemia/reperfusion (I/R) groups. Mitochondrial respiration rate was measured as described in Methods using 5 mM glutamate plus 5 mM malate as substrates. The blue trace represents oxygen concentration (nmol/mL), and the red trace represents oxygen flux. I20/R30, I30/R30, I40/R30, and I60/R30 refer to different ischemia durations (respectively, 20, 30, 40, and 60 minutes) following by 30-minute reperfusion. V₀: routine respiration rate in the presence of 0.5 mg/mL of mitochondria and substrates; V₃: state 3 respiration rate in the presence of 1 mM ADP; V_3+cyt c: state 3 respiration rate in the presence of 32 μM cytochrome c; V_{ATR (+cyt c)}: respiration rate in the presence of 0.12 mM atractyloside and 32 μM cytochrome c.