Figure 3.

Mutant NPII Is Confined within ERACs in AVP Neurons of FNDI Mice

(A–C) Immunofluorescence staining for mutant (green) and normal (magenta) NPII, as well as DAPI (blue) in the SON of FNDI mice. Higher magnification images of the boxed areas in the left panels are shown at right. Scale bars: 50 μm (left panels) and 10 μm (right panels).

(D–I) Immunoelectron microscopic analysis of mutant NPII (10 nm gold particles) and normal NPII (15 nm gold particles) in AVP neurons from the SON (D–G) and posterior pituitary (H and I) of FNDI mice. (D) An ERAC and a vesicle containing neurosecretory granules are shown. (G) Aggregates in the ER lumen. Higher magnification images of the boxed areas in D and H are shown in E, F, and I, respectively. The white arrowheads in F, G, and I indicate neurosecretory granules containing AVP. m: mitochondria. Scale bars: 100 nm (D, E, F, and I) and 500 nm (G and H).

See also Figure S3.