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. 2020 Oct 7;23(10):101648. doi: 10.1016/j.isci.2020.101648

Figure 4.

Figure 4

ERACs Are Surrounded by Membranes that Express BiP and LC3

(A–C) Immunofluorescence staining for mutant NPII (green) and BiP (magenta) in the SON of FNDI mice. Higher magnification images of the boxed areas in the left panels are shown at right. Scale bars: 50 μm (left panels) and 10 μm (right panels).

(D and E) Immunoelectron microscopic analysis for BiP (10 nm gold particles) and normal NPII (15 nm gold particles) in AVP neurons from the SON of FNDI mice. Higher magnification image of the boxed area in D is shown in E. m: mitochondria, Gol: Golgi apparatus. Scale bars: 500 nm (D) and 100 nm (E).

(F–H) Immunofluorescence staining for GFP-LC3 (green) and mutant NPII (magenta) in the SON of FNDI/GFP-LC3 mice. Higher magnification images of the boxed areas in the left panels are shown at right. Scale bars: 50 μm (left panels) and 10 μm (right panels).

(I) Immunoelectron microscopic analysis of GFP-LC3 (12 nm gold particles, white arrowheads) in AVP neurons from the SON of FNDI/GFP-LC3 mice. A small portion of an ERAC is shown. Scale bar: 100 nm.

See also Figures S3–S5.