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. 2020 Oct 21;10:17912. doi: 10.1038/s41598-020-74960-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design of the three-way comparison study. Schematic experimental design to assess the total mutagenesis and knock-in rate induced in embryos or live pups obtained from wild-type C57BL/6J zygotes or maternally supplied Cas9 C57BL/6J zygotes after microinjection or electroporation of CRISPR reagents. ssODN⁺: optional inclusion of ssODN in the electroporation mix.