Figure 2.

Proof-of-principle and conditions optimization experiments. (a) Table summarizing editing of Gene A after pronuclear microinjection (PNI) or electroporation (EP) of CRISPR reagents into wild-type C57BL/6J embryos with various concentrations of Cas9 protein. (b) Total mutagenesis rate at Gene A locus. (c) Rate of homology directed repair at Gene A locus. The values presented in table (a) are total numbers across all replicates, whereas the numbers from individual replicate experiments are shown in (b) and (c).