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. 2020 Oct 21;10:17884. doi: 10.1038/s41598-020-74927-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of internal and external control gene on extraction efficiency normalization. Calibration curves plot nominal concentration against cycle threshold for trials with or without normalization (a) by internal (b) or external control genes (c). We compared the accuracy of quality control (QC) samples prepared from pooled blood with or without normalization by an internal (d) or external control gene (e). The accuracy of QC samples prepared from the blood of eight different donors was assessed for both methods with normalization by internal or external control genes (f). The correlation between the DNA concentration in eluate and the accuracy of QC samples prepared from the blood of eight different donors for the method with normalization based on the internal control gene (g).