Table 1.
Validation of volume-based qPCR method for transgene detection in human blood.
| Nominal concentration (copies/μL blood) | 1st day | 2nd day | 3rd day | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Mean observed concentration (copies/μL blood) | Relative error (%) | Coefficient of variation (%) | Mean observed concentration (copies/μL blood) | Relative error (%) | Coefficient of variation (%) | Mean observed concentration (copies/μL blood) | Relative error (%) | Coefficient of variation (%) | |
| 200,000 | 218,066.2 | 9.0 | 5.4 | 196,127.5 | − 1.9 | 6.9 | 190,866.7 | − 4.6 | 9.0 |
| 2,000 | 1895.1 | − 5.2 | 10.3 | 1803.6 | − 9.8 | 7.4 | 1852.4 | − 7.4 | 8.1 |
| 7.5 | 8.1 | 7.4 | 7.0 | 8.2 | 9.4 | 4.4 | 7.3 | − 2.7 | 5.7 |
| Slope | − 3.35 | − 3.39 | − 3.33 | ||||||
| intercept | 8.35 | 8.25 | 8.37 | ||||||
| PCR efficiency (%) | 98.8 | 97.2 | 99.5 | ||||||
| R2 | 1.000 | 0.999 | 1.000 | ||||||