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. 2020 Oct 21;10:17910. doi: 10.1038/s41598-020-74609-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The overexpression of CwlO improved the viability and rod morphology of the ugtP lytE mutant. (A) Spot plate assay for cells with an additional cwlO gene under the control of a xylose promoter. BSB1 ΔugtP ΔlytE cells overexpressing CwlO (+xylose) were viable on PAB. (B) Fluorescence microscopy for BSB1 ΔugtP ΔlytE cells overexpressing CwlO recovered partially the rod-shape morphology however cells were still bent. X− without xylose, X+ with xylose. Scale bar 3 µm.