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. 2020 Aug 19;7(20):2001019. doi: 10.1002/advs.202001019

Figure 5.

Figure 5

SNAI1 is critical for mesoderm induction and HPC generation. A) The morphology of cells derived from WT and SNAI1−/−, SNAI2−/−, ZEB2−/− hESCs at day 2 of hematopoietic differentiation. B) The expression of representative mesenchymal genes in the cells derived from WT and SNAI1−/−, SNAI2−/−, ZEB2−/− hESCs at day 2 of hematopoietic differentiation. C) ChIP‐qPCR analysis of the occupancy of SNAIL1 at the promoter region of TBX20, BMP2, and NRP1. D) The box plot showing the expression of mesenchymal genes in the cells derived from WT and SNAI1−/− hESCs at day 2 of hematopoietic differentiation. E) Comparison of “mesoderm development”‐associated gene expression between the cells differentiated from WT and SNAI1−/− hESCs at day 2 of hematopoietic differentiation. F) The percentage of CD43+ HPCs derived from WT and SNAI1−/− hESCs. G) The relative mRNA expression level of representative mesenchymal genes in the cells derived from WT and SNAI1−/− hESCs with or without CHIR treatment. H) The percentage of CD43+ HPCs derived from WT and SNAI1−/− hESCs with or without CHIR treatment. NS, not significant, *P < 0.05, **P < 0.01, and ***P < 0.001.