Figure 3.
Involvement of m6A modification in regulation of FOXO3 mRNA in the luteinized GCs of the controls but not PCOS patients. A, mRNA levels of PCOS‐associated genes (AMH, AR, FOXO1 and FOXO3) in the luteinized GCs from the controls and PCOS patients. B, Expression of the methyltransferases (METTL3 and METTL14) and demethylases (FTO and ALKBH5) in the luteinized GCs of controls and PCOS patients. C‐J, The effects of selectively knockdown of the methyltransferases or demethylases on the expression of FOXO1, FOXO3 and SBK1 in the luteinized GCs of the controls and PCOS patients. GCs of the controls and PCOS patients were collected, cultured and subjected to METTL3‐siRNA (C,D), METTL14‐siRNA (E,F), FTO‐siRNA (G,H) or ALKBH5‐siRNA (I,J), respectively. qRT‐PCR analysis was performed to examine the mRNA levels of FOXO1, FOXO3 and SBK1. Data were expressed as fold changes relative to the blank controls. Bars represent means ± SD, n = 5. *P < 0.05 vs the blank controls