FIGURE 1.

Kinome‐wide shRNA library screen identifies PDPK1 as putative target regulating the survival of PCa cells. A, Kinase shRNA screen scatter plot. Z‐scores are plotted on the y‐axis against 3109 corresponding shRNAs on the x‐axis. The red and green circled dots represent shRNA hits, which the former inhibited cell proliferation and the latter promoted cell proliferation. B, Protein‐protein interaction network of the PDPK1 target proteins. C and D, PDPK1 is expressed in a subset of PCa cells and RWPE‐1 non‐transformed prostate epithelial cells. PDPK1 mRNA expression was evaluated by qPCR with GAPDH as housekeeping gene. The level of PDPK1 protein expression was detected by immunoblotting with β‐actin as loading control