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. 2020 Sep 14;24(20):12188–12198. doi: 10.1111/jcmm.15876

FIGURE 2.

FIGURE 2

Depletion of endogenous PDPK1 induces tumour‐specific cell death in PCa cells. A, Effective PDPK1 knock‐down was achieved by two independent shRNA constructs targeting PDPK1 (PDPK1‐si1 and PDPK1‐si2). Lysates were harvested at 72 h post‐lentiviral transduction and analysed by immunoblotting. B and C, PDPK1 depletion selectively inhibited the proliferation and induced apoptosis in AR‐negative DU145 and PC3 PCa cells but not in AR‐positive LNCaP or RWPE‐1 non‐transformed prostate epithelial cells. Cell viability and apoptosis were measured using CellTiter‐Glo® assay and annexin V/7‐AAD flow cytometry at 72 h post‐transduction. D, Depletion of endogenous PDPK1 induced caspase 3 and 9 activities. Caspase 3, 8 and 9 activities were evaluated by CaspaseGlo assay at 72 h post‐transduction. Bars represent means ± SD of three independent experiments. (*) indicates statistical significance compared with NS control cells (P < .01, Student's t test)