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Depletion of endogenous PDPK1 reduces SGK3 phosphorylation. PDPK1 depletion down‐regulated SGK3 phosphorylation in AR‐negative DU145 and PC3 cells, but not in AR‐positive LNCaP PCa cells or RWPE‐1 non‐transformed prostate epithelial cells. The protein expression and phosphorylation of AKT, SGK1 and SGK3 Lysates were analysed by immunoblotting with β‐actin served as loading controls
