FIGURE 4.

PDPK1 regulates the survival of DU145 and PC3 cells via SGK3 signalling pathway. Ectopic expression of a constitutively active SGK3 (S486D) reduced apoptotic cell death induced by PDPK1 knock‐down in DU145 cells and PC3 cells. A, The constitutively active SGK3 S486D plasmid was cotransfected with either NS or PDPK1 targeting shRNAs into DU145 and PC3 PCa cells. Whole cell protein lysates were harvested 72 h post‐transfection and analysed by immunoblotting. B, The apoptotic cell death was detected by annexin V/7‐AAD flow cytometry. Bars represent means ± SD of three independent experiments. (*) indicates statistical significance compared with Vec control cells (P < .01, Student's t test)