Figure 4.

Deficiency of UGDH by siRNA decreased cell migration and wound healing ability in ovarian cancer cell lines. A, Left panel: Knockdown efficiency by siUGDH was measured by immunoblotting. UGDH siRNA efficiently decreases ~90% of the protein level in both TOV21GLI and TOV21GHI cells. Middle: Representative images of the migrated cells at 10× magnification using an optical microscope. Right panel: transwell migration assay was performed to monitor the effect of siUGDH on the cell migration ability. After seeding the cells in the transwell insert for 18 h, migrated cells were fixed and stained with crystal violet for quantification. Absorbance values were normalized to the value of TOV21G^LI control cells. Data are represented as the mean ± SEM. ***, P < .001 compared to control ovarian cancer cells. B, Wound healing assay showed the same trend as the transwell assay. After treatment with siRNA, the wound healing of ovarian cancer cells was monitored and photographed at 0, 4, 8 and 12 h by an using optical microscope. The healing areas at certain times were quantified by AxioVision 4.8 software. The amounts of healing area were normalized to the amount at 0 h. Data are represented as the mean ± SEM. ***, P < .001 compared to control ovarian cancer cells. C, Knockdown efficiency of siUGDH in A2780 and HeyA8 cells was monitored by immunoblotting. Transwell migration assay was applied to monitor the effects of siUGDH in A2780 and HeyA8 cells