Figure 3.
Mesenchymal stem and progenitor cells (MSPC) characterization and multilineage differentiation analysis. The specific characteristics of the human primary MSPCs used were demonstrated according to (A) the positive expression of CD73, CD90, CD105, and the negative expression of CD14, CD19, CD34, CD45, and HLA-DR. The percentage of positive stained cells are presented (mean ± SD; n = 3). The multilineage differentiation potential was proved for all primary cultures by (B) the relative expression of alkaline phosphatase (ALP), osteocalcin, and osteopontin for the osteogenic differentiation (mean ± SD; n = 7; measured in quadruplicate), (C) Alcian blue staining and the expression of aggrecan for the chondrogenic differentiation (mean ± SD; n = 7; measured in quadruplicate), and (D) the oil red O staining of lipid droplets for the adipogenic lineage. One representative picture is shown. **: p < 0.01; *: p < 0.05.