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. 2020 Oct 8;13(19):4454. doi: 10.3390/ma13194454

Table 5.

Effects of NCA on selected cell lines in in vitro assays.

Compound Relation Value Unit Standard Assay Cell Line Source
BA > 10 µmol/L IC50 Cytotoxicity against human cells after 48 h by SRB assay BT-549 [164]
> 10 µmol/L IC50 A549 [164]
> 10 µmol/L IC50 SK-MEL-2 [164]
> 50 µmol/L IC50 Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 h by Griess assay BV-2 [164]
> 10 µmol/L IC50 Cytotoxicity against human SKOV3 cells after 48 h by SRB assay SK-OV-3 [164]
CinA = 64 µmol/L IC50 Anticancer activity against human cells after 48 h by MTT assay A-375 [165]
= 108 µmol/L IC50 MCF7 [165]
= 91 µmol/L IC50 ACHN [165]
= 87 µmol/L IC50 A549 [165]
= 114 µmol/L IC50 HT-29 [165]
> 100 µmol/L IC50 Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 h in presence of LPS by Griess assay BV-2 [165]
p-CA > 100 µmol/L IC50 Cytotoxicity against human SK-MEL-28 cells after 72 h by MTT assay SK-MEL-28 [166]
> 100 µmol/L IC50 Cytotoxicity against human A549 cells after 72 h by MTT assay A549 [166]
> 10 µmol/L IC50 Cytotoxicity against human SKOV3 cells after 48 h by SRB assay SK-OV-3 [164]
= 10 µmol/L IC50 Cytotoxicity against human BT549 cells after 48 h by SRB assay BT-549 [164]
> 2000 µmol/L IC50 Antiproliferative activity against human U937 cells assessed as incorporation of methyl-3H-thymidine after 12 h by scintillation counting U-937 [167]
> 200 µmol/L IC50 Antitumor activity against KB cells by MTT assay KB [168]
= 82 µmol/L IC50 Cytotoxicity against human LoVo cells after 72 h by MTT assay LoVo [166]
> 10 µmol/L IC50 Cytotoxicity against human A549 cells after 48 h by SRB assay A549 [164]
> 100 µmol/L IC50 Cytotoxicity against human PC3 cells after 72 h by MTT assay PC-3 [166]
> 10 µmol/L IC50 Cytotoxicity against human SK-MEL-2 cells after 48 h by SRB assay SK-MEL-2 [164]
CFA = 317 µmol/L IC50 Antiproliferative activity against human U937 cells assessed as incorporation of methyl-3H-thymidine after 12 h by scintillation counting U-937 [167]
= 700 µmol/L IC50 Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 h by luminescence-based ATP assay A549 [167]
= 6.4 µmol/L IC50 Antiproliferative activity against human MOLM13 cells by CellTiter-Glo assay MOLM-13 [169]
= 500 µmol/L IC50 Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 h by FMCA assay A549 [167]
= 30 µmol/L IC50 Cytotoxicity against human HCT116 cells after 96 h by MTT assay HCT-116 [170]
> 10 µmol/L IC50 Antiproliferative activity against human MV4-11 cells by CellTiter-Glo assay MV4-11 [169]
= 76 µmol/L IC50 Neuroprotection against amyloid beta (25 to 35)-induced cell death in rat PC12 cells pre-incubated for 3 h followed by amyloid beta addition measured after 24 h by MTT assay PC-12 [171]
- - - IC50 Activity against hydrogen peroxide induced DNA damage in Jurkat T cells Jurkat [172]
= 27 µmol/L IC50 Cytotoxicity against human HT-29 cells after 96 h by MTT assay HT-29 [170]
= 700 µmol/L IC50 Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 h by MTT assay A549 [167]
> 550 µmol/L IC50 Cytotoxicity against African green monkey Vero cells assessed as [3H]-hypoxanthine incorporation after 48 h Vero [173]
> 550 µmol/L IC50 Cytotoxicity against human MCF7 cells assessed as [3H]-hypoxanthine incorporation after 48 h MCF7 [173]
= 129 µmol/L IC50 Cytotoxicity against human AGS cells after 96 h by MTT assay AGS [170]
= 940 µmol/L IC50 Anticomplement activity in rabbit erythrocytes assessed as concentration required for 50% hemolytic inhibition by alternative pathway pre-treated for 10 min with normal human serum followed by erythrocyte addition measured after 30 min by spectrophotometric method Erythrocyte [174]
= 44.0 µg/mL IC50 Antiallergic activity in Ca(2+)-stimulated differentiated human HeLa cells assessed as inhibition of cys-leukotriene release after 6 days by ELISA HeLa [175]
= 0.002 µmol/L IC50 Antiproliferative activity against human T47D cells after 5 days by MTT assay T47D [176]
= 750 µmol/L IC50 Anticomplement activity in sheep erythrocytes assessed as concentration required for 50% hemolytic inhibition by classic pathway pre-treated for 10 min with guinea pig serum followed by erythrocyte addition measured after 30 min by spectrophotometric method Erythrocyte [174]
> 100 µmol/L IC50 Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 h by WST-1 assay LNCaP [177]
> 100 µmol/L IC50 Antiproliferative activity against human A549 cells after 72 h by MTT assay A549 [178]
> 10 µmol/L IC50 Antiproliferative activity against human MOLM14 cells by CellTiter-Glo assay MOLM-14 [169]
RA = 40.4 % Inhibition Inhibition of Jurkat cell activation assessed as blocking of T-cell antigen receptor-induced IL-2 expression at 10 µmol/L by luciferase assay Jurkat [179]
= 50 % Inhibition Inhibition of Jurkat cell activation assessed as blocking of T-cell antigen receptor-induced IL-2 expression at 30 µmol/L by luciferase assay Jurkat [179]
- - - Activity Cytotoxicity against human HepG2 cells up to 20 µmol/L after 24 h by MTS assay HepG2 [180]
= 71 % Activity Inhibition of cell proliferation of human U251 cells assessed as cell viability at 100 µmol/L after 72 h by SRB assay U-251 [181]
= 27 % Inhibition Inhibition of Wnt/beta-catenin signaling pathway in human HEK293 cells at 20 µmol/L after 24 h by dual luciferase reporter gene assay relative to vehicle-treated control HEK293 [182]
- - - Activity Cytoprotection against phototoxicity in human NHDF cells assessed as increase in cell viability at 3.9 to 31.3 µmol/L preincubated for 60 min followed by 7.5 J/cm2 UVA irradiation and measured after 24 h by neutral red uptake assay NHDF [183]
- - - Activity Cytoprotection against phototoxicity in human HaCaT cells assessed as increase in cell viability at 3.9 µmol/L preincubated for 60 min followed by 10 J/cm2 UVA irradiation and measured after 24 h by neutral red uptake assay HaCaT [183]
- - - Activity Cytoprotection against phototoxicity in human NHDF cells assessed as increase in cell viability at 3.9 to 31.3 µmol/L preincubated for 60 min followed by 150 mJ/cm2 UVB irradiation and measured after 24 h by neutral red uptake assay NHDF [183]
- - - Activity Cytoprotection against phototoxicity in human HaCaT cells assessed as increase in cell viability at 3.9 to 31.3 µmol/L preincubated for 60 min followed by 10 J/cm2 UVA irradiation and measured after 24 h by neutral red uptake assay HaCaT [183]
= 2.9 µmol/L IC50 Antiproliferative activity against human cells by CellTiter-Glo assay MOLM-13 [169]
> 10 µmol/L IC50 MV4-11 [169]
= 7.1 µmol/L IC50 MOLM-14 [169]
= 55 µmol/L CC50 Cytotoxicity against human MT4 cells by MTT method MT4 [184]
D-ChA = 39.7 µmol/L IC50 Concentration of compound required to reduce MT-4 cell viability by 50% MT4 [185]
= 35.5 µmol/L IC50 Compound was evaluated for the cytoprotection of CEM-SS cells by XTT cytoprotection assay through the NCI AIDS Screen CEM-SS [185]
L-ChA = 20.1 µmol/L IC50 CEM-SS [185]
= 45 µmol/L IC50 Concentration of compound required to reduce MT-4 cell viability by 50% MT4 [185]
D-ChA = 111 µmol/L CC50 Cytotoxicity against human MT4 cells by MTT assay MT4 [186]

SRB—sulforhodamine B; MTT—3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; XTT—(2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide).