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. 2020 Oct 7;11:561317. doi: 10.3389/fmicb.2020.561317

TABLE 2.

Kinetic parameters of the β-lactamases KPC-55 and KPC-2.

	Hydrolysis activity^a

	KPC-55			KPC-2

Substrate	k_cat (s^–1)	K_m (μM)	k_cat/K_m (s^–1 μM^–1)	k_cat (s^–1)	K_m (μM)	k_cat/K_m (s^–1 μM^–1)
Ampicillin	0.06 ± 0.05	0.03 ± 0.01	1.89 ± 1.81	5.31 ± 2.8	1.03 ± 0.38	4.98 ± 0.77
Aztreonam	27.89 ± 5.20	1.50 ± 0.31	18.58 ± 0.36	2.56 ± 3.23	1.17 ± 1.29	1.85 ± 0.50
Ceftazidime	0.20 ± 0.33	0.40 ± 0.11	0.45 ± 0.74	0.02 ± 0.003	0.28 ± 0.09	0.07 ± 0.02
Imipenem	1.99 ± 0.64	0.13 ± 0.002	15.14 ± 4.61	0.41 ± 0.30	0.03 ± 0.02	37.48 ± 47.21
Meropenem	1.34 ± 0.05	0.02 ± 0.01	80.49 ± 35.18	0.09 ± 0.03	0.02 ± 0.02	5.19 ± 2.93

^aData are the means of three independent determinations.