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. 2020 Oct 8;11:561370. doi: 10.3389/fendo.2020.561370

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Copyright © 2020 Stelcer, Milecka, Komarowska, Jopek, Tyczewska, Szyszka, Lesniczak, Suchorska, Bekova, Szczepaniak, Ruchala, Karczewski, Wierzbicki, Szaflarski, Malendowicz and Rucinski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative expression levels of the adropin precursor gene ENHO and the adropin receptor GPR19 in HAC15 cells. (A) Real time qPCR study in untreated (control) cells and cells incubated with ACTH, forskolin, and adropin for 24 h (N = 4/group). Statistical differences were determined by Mann–Whitney U non-parametric test compared to controls. Both the expression of ENHO and GPR19 did not differ significantly from the control group. (B) GPR19 immunoreactivity in untreated HAC15 cells. The reaction was detected in cytoplasmic vesicles and cell membranes (indicated by white arrows). (C) Semi-quantitative analysis of GPR19 expression at protein level using western blot method.