Figure 4.

Immunomodulatory effect of exosomes produced by KEL cells on human THP-1 monocytes under hypoxia condition. THP-1 cells were cocultured with exosomes excreted by KEL cells under hypoxia or normoxic condition for 24 h. Then, mRNA or protein expression levels of TNF-α, IL-1β, IL-10, and CD206 were detected. (a) The statistical assay of mRNA expression of TNF-α, IL-1β, IL-10, and CD206 in THP-1 cells. (b) ELISA assayed the protein levels of TNF-α, IL-1β, and IL-10 in the supernatants. The results represent three independent experiments. N: normoxia; Hx: hypoxia. ^∗P < 0.05, ^∗∗P < 0.01.