FIGURE 3.

Effect of acacetin on myocardial apoptosis in mouse hearts with doxorubicin. A, Representative ventricular sections stained with TUNEL and Dapi to determine apoptotic cardiomycytes in mice treated with vehicle (control), doxorubicin or doxorubicin plus acacetin. B, Percentage of apoptotic cardiomyocytes in mouse hearts treated with vehicle (control), doxorubicin or doxorubicin plus acacetin. C, Western blots of Bcl‐2, Bax and cleaved caspase‐3 in ventricular tissues of mice administered with vehicle (Control), doxorubicin (Dox) and acacetin prodrug plus doxorubicin. D, Summarized relative levels of Bcl‐2, Bax and cleaved caspase‐3 expression in ventricular tissues of mice from Western blots as shown in C (n = 5 individual experiments, ^* P < 0.05, ^** P < 0.01 vs control; ^# P < 0.05, ^## P < 0.01 vs doxorubicin alone)