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. 2020 Sep 1;24(20):11922–11935. doi: 10.1111/jcmm.15812

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© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of SM‐BFRE on the migration of laryngeal carcinoma cells. (A and C) Micrographs of two laryngeal carcinoma cells at 0, 12 and 24 h after treatment with SM‐BFRE (0, 5, 10 and 20 μg/mL), respectively, and relative wound surface area in wound healing assay was calculated by ImageJ software. (B and D) The expression of MMP‐9 protein was detected by Western blot assay, and the relative expression was calculated by Image Lab software. β‐actin was used as a control. *P < .05, **P < .01 or ***P < .001 compare to control (0 μg/mL served as control)