FIGURE 3.

Effect of ferrostatin‐1 (FER‐1) on reactive oxygen species (ROS) generation in cisplatin‐damaged House Ear Institute‐Organ of Corti 1 (HEI‐OC1) cells. A, The experimental workflow. The HEI‐OC1 cells were pre‐treated with 30 μmol/L FER‐1 or 3 μmol/L RSL3 for 2 h and then treated with or without 30 μmol/L cisplatin for another 24 h, or treated with 30 μmol/L cisplatin alone for 24 h. B, Representative images of HEI‐OC1 cells stained by DCFH‐DA and MitoSox‐Red. Scale bar, 20 µm. C and D, Measurement and quantification of intracellular and mitochondrial ROS levels by flow cytometry using DCFH‐DA and MitoSox‐Red fluorescent probes. The data are shown as mean ± SEM. of three independent experiments. ****P < 0.0001 and n.s. no significant vs the control group; ^#### P < 0.0001 vs the cisplatin group