FIGURE 7.

Effect of ferrostatin‐1 (FER‐1) on cisplatin‐induced cochlear hair cell damage. A, The experimental workflow. Cochlear explants were treated with 30 μmol/L cisplatin alone for 24 h, or pre‐treated with 30 μmol/L FER‐1, 3 μmol/L RSL3 or 3 μmol/L RSL3 + 5 mmol/L NAC for 2 h and addition of 30 μmol/L cisplatin for 24 h followed by 3 d recovery. B, Representative images of cochlear hair cells stained by myosin 7a (red). Scale bar, 20 µm. C, Quantification of myosin 7a‐positive hair cells in the apical, middle, and basal turns of different groups. The data are shown as mean ± SEM. *P < 0.05, ****P < 0.0001 and n.s. no significant vs the control group; ^#### P < 0.0001, n = 12 cochlear explants from three independent experiments