FIGURE 8.

Effects of ferrostatin‐1 (FER‐1) on reactive oxygen species level and 4‐HNE in cisplatin‐damaged cochlear hair cells. A, The experimental workflow. Cochlear explants were treated with only 30 μmol/L cisplatin (24 h), 3 μmol/L RSL3 (26 h), or pre‐treated with 30 μmol/L FER‐1 or 3 μmol/L RSL3 for 2 h and addition of 30 μmol/L cisplatin for 24 h followed by 3 d recovery. B, Representative images of cochlear (middle turn) hair cells stained by DCFH‐DA (green), 4‐HNE (red) and myosin 7a (grey). Scale bar, 20 µm. C and D, Relative fluorescence intensity of DCFH‐DA and 4‐HNE. The data are shown as mean ± SEM. ***P < 0.001, ****P < 0.0001 and n.s. no significant vs the control group; ^### P < 0.001, ^#### P < 0.0001 vs the cisplatin group, n = 6 cochlear explants from three independent experiments