Skip to main content
. 2020 Sep 11;24(20):12094–12106. doi: 10.1111/jcmm.15846

Figure 5.

Figure 5

Overexpression of LINC00470 up‐regulated the expression of MYC by regulating miR‐134. A, MYC was predicted to be a direct target of miR‐134. B, The luciferase activity of miR‐134 mimics and MYC‐Wt co‐transfected cells was inhibited, while the luciferase activity of miR‐134 mimics and MYC‐Mut co‐transfected cells remained unchanged. C,D, miR‐134 mimics reduced the expression of MYC at both the mRNA and protein levels in U87 and U251 cells. GAPDH was the internal control. E,F, LINC00470 overexpression up‐regulated the expression of MYC at both the mRNA and protein levels in U87 and U251 cells. When miR‐134 mimics were transfected into LINC00470‐overexpressing cells, the up‐regulation of MYC expression was offset. GAPDH was the internal control. The data shown represent the mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. NC, negative control