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. 2020 Sep 11;24(20):12164–12175. doi: 10.1111/jcmm.15864

Figure 5.

Figure 5

Exosomal miR‐200c regulates the activation of β‐catenin by targeting SOX1. A, Predicted binding sites and corresponding mutant sites in the 3' UTR of SOX1 mRNA (WT, wild‐type; MUT, mutant type). B, Relative SOX1 reporter activity in melanocytes co‐transfected with miR‐200c and luciferase reporters. C, Western blot analysis of SOX1 protein expression in melanocytes transfected with miR‐200c mimics. D, SOX1 and β‐catenin protein levels in melanocytes cocultured with NHEK or VLK exosomes for 72 h. E, Western blot analysis of SOX1 and β‐catenin expression in melanocytes transfected with miR‐200c inhibitor. F, Western blot analysis of SOX1, β‐catenin expression in melanocytes treated with corresponding exosomes and plasmids. G, Western blot analysis of the nuclear‐β‐catenin expression in melanocytes treated with corresponding exosomes and plasmids. Results are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.005