FIGURE 1.

Melatonin enhanced dabrafenib‐mediated cell proliferation inhibition. A, Human anaplastic thyroid cancer cells were treated with melatonin at the indicated doses. After 48 h, cell viability was determined by CCK‐8 assay. B, Human anaplastic thyroid cancer cells were treated with the indicated doses of dabrafenib (Da) alone or combined with melatonin (Mel) (1mM) for 48 h, and cell viability was examined by CCK‐8 assay. C and D, SW1736 and OCUT1 cells were treated with Mel (1 mmol/L) or Da (0.1 μmol/L) alone or their combination. The colony formation results were photographed (C), and their relative numbers were counted (D). E, The expression of AKT signalling–related proteins p‐AKT and AKT in anaplastic thyroid cancer cells with indicated treatment was respectively detected by Western blot assays. Data were presented as mean ± SD of three independent experiments. The level of significance was indicated by ***P < .001, **P < .01