FIGURE 1.

PB2 inhibits OS cell proliferation and induces apoptosis. A, OS cell lines (143B, MNNG, SJSA and MG‐63) and normal human osteoblast cells (hFOB1.19) were treated with PB2 (30‐100 μmol/L) for 24, 48 or 72 h, and cell proliferation was determined using CCK‐8 assays. B,C, Macrograph of colony formation for 143B and MNNG cells (n = 3, ^*,&,δ P < .001 for PB2 vs NC). D, PCNA protein levels assessed by Western blotting. E, The mRNA expression levels for PCNA, Caspase‐3, Caspase‐9, Bax, Bcl‐2, PI3K and AKT determined by qRT‐PCR (n = 3, ^* P < .05 for PB2 vs NC)